The Role of ZFH-04269, Pilicides ec240, and Mannosides as FimH Adhesins Inhibitor of Uropathogenic Escherichia coli (UPEC) and Multiplex Real-Time PCR as a Potential Method in Diagnosing Urinary Tract Infections

Ignatius Ivan, Harvey Sudharta, Nicolas Daniel Widjanarko, Maureen Miracle Stella, Kevin Tandarto, Mariani Santosa


Urinary tract infections (UTIs) can be difficult to diagnose and therefore a quicker and cheaper method but still having a high reliability result is needed. Multiplex real-time PCR can diagnose UTI better, as it has a high accuracy and time-efficiency. In other hand, as many infection is caused by bacterial attachment to cells, anti-adhesion therapeutics can be an option for treatment methods. Our objective is to analyse whether real-time multiplex PCR and anti-adhesive therapeutics can become the best methods for diagnosis and treatment options respectively. Results showed that it is feasible to identify uropathogens with more than 90% accuracy by using multiplex real-time PCR; while modified isoquinolone-mannoside is proved to have the highest FimH affinity and potency of any antagonists reported to date. Finally, we conclude that multiplex real-time PCR and the use of ZFH-04269, pilicides ec240, and mannosides as FimH adhesins inhibitor might be applicable as novel diagnostic method and treatment for UTIs.


Escherichia coli, UTI, Anti-adhesive, Multiplex Real-time PCR

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